Figure 2: HK2 and PKM2 glycolytic isozyme expression promotes liver growth.
From: PPARγ contributes to PKM2 and HK2 expression in fatty liver
(a) Immunoblot analysis of PKM2 and HK2 protein expression in livers of mice, 7 days after transduction with indicated adenoviruses. (b) Liver weight relative to body weight of mice transduced with indicated adenoviruses. Data are means±s.e.m., n=3–8 (P<0.05 (a) versus GFP/WT; (b) versus adenoHK2/WT; (c) versus adenoPKM2/WT; (d) versus GFP/AlbCre;PTENf/f;Akt2−/−; 2-tailed, unpaired Student's t test). (c) Hepatic lactate levels of random-fed mice of indicated genotypes 7 days after transduction with HK2 adenovirus. Data are means±s.e.m., n=3 (P<0.05 (a) versus GFP; 2-tailed, unpaired Student's t test). (d) H&E-stained sections of WT and AlbCre;PTENf/f;Akt2−/− livers transduced with GFP or HK2 adenoviruses. Bar represents 100 μm. (e) Increased proliferation rate of hepatocytes induced by HK2 overexpression in the livers of mice of indicated genotypes. BrdU+ nuclei and total number of nuclei were scored in the livers of adenovirus-transduced mice 1 day after administration of BrdU in drinking water. Data presented as a ratio of BrdU+ to total number of nuclei ±s.e.m., n=3–8 (P<0.05 (a) versus GFP; 2-tailed, unpaired Student's t test).
