Figure 1: Cell shape changes induce deformation and orientation of the nucleus.
From: Spatial coordination between cell and nuclear shape within micropatterned endothelial cells
(a) DIC images of single ECs constrained on 1,600 μm2 FN-coated micropatterns of various shapes (from CSI ∼1 to ∼0.26). The nucleus, stained in blue with DAPI and superimposed on DIC images, shows NSI ranging from 0.95 in circular-shaped cells to 0.73 in highly elongated cells. The scale bars correspond to 10 μm. (b) Evolution of NSI as a function of CSI for a wide range of SAs (from 600 to 2,000 μm2). To highlight the role of the spreading area, curves for 600 and 2,000 μm2 were fitted with an asymptotic function, in red and purple, respectively. Data are given as mean±s.d. with n=15. (c) Angular graphs, superimposed on micropattern drawings in grey, show the different orientations experienced by nuclei in response to cell shape changes for various SAs (from 600 to 2,000 μm2). Data are given as mean±s.d. .
