Figure 2: The remodelling of the nucleus is mediated by cell spreading area and actomyosin activity.
From: Spatial coordination between cell and nuclear shape within micropatterned endothelial cells
(a) Immunostained images of ECs at different spreading rates (from left to right: 0.15, 0.35, 0.50, 0.80 and 1) on elongated micropatterns with a constant surface area (1,600 μm2). The nucleus is stained in blue with DAPI, actin filaments in green with Alexa 488-phalloidin and focal adhesions in red with vinculin. The scale bar corresponds to 12 μm. (b) Evolution of the NSI of ECs as a function of their spreading rate on elongated micropatterns with a constant surface area (1,600 μm2). The red line corresponds to the best-fit linear regression. (c) Evolution of the NSI of ECs plated on 1,600 μm2 elongated micropatterns (CSI=0.26) in response to various pharmacological agents. NSI for control cells is presented in black. Data are given as mean±s.d. (12≤n≤15). (d) Effect of cytoskeletal inhibitors on the angular distribution of nucleus in 1,600 μm2 elongated-shaped ECs (CSI=0.26). Data are given as mean±s.d. (12≤n≤15).
