Figure 6: Cell elongation leads to chromatin condensation and affects cell proliferation.
From: Spatial coordination between cell and nuclear shape within micropatterned endothelial cells
(a) Successive changes of the level of chromatin condensation as the NSI decreases. Intensities of DNA staining were digitized in 256 bits and colour coded (from high to low: white, purple, red, orange, yellow, green, light blue and dark blue). Highly condensed domains show higher fluorescence intensity with respect to the less condensed ones. (b) Evolution of the mean average spatial density of the nucleus as a function of the CSI. (c) Elongated cells (CSI∼0.26, light grey bar) were treated with thrombin (hatched bar) and blebbistatin (cross-hatched bar). The average spatial density quantified by DAPI staining was compared with circular-shaped cells (dark grey bar). (d) Evolution of the DNA synthesis in response to cell shape changes (1≥CSI≥0.26). Data are given as mean±s.d. with n≥12 for b, c and 69≤n≤76 for d.
