Figure 3: Properties of the induced pluripotent stem cell (iPSC)-derived midbrain dopaminergic (DA) neurons. | Nature Communications

Figure 3: Properties of the induced pluripotent stem cell (iPSC)-derived midbrain dopaminergic (DA) neurons.

From: Parkin controls dopamine utilization in human midbrain dopaminergic neurons derived from induced pluripotent stem cells

Figure 3

(a–h) P002 iPSC-derived neuronal cultures were co-stained for TH and the midbrain markers engrailed-1 (En-1) (a) and FoxA2 (b), the DA markers AADC (c), dopamine transporter (DAT) (d) and VMAT2 (e), the synaptic markers synaptophysin (f) and NR1 (g), and the marker for mature neurons MAP2 (h). Bars, 10 μm. (i–n) These neurons fired spontaneous APs (i) and evoked APs (j), had voltage-gated K+ and Na+ currents (k), N-methyl-D-aspartic acid (NMDA)-gated currents (l), γ-aminobutyric acid (GABA)-gated currents (m) and spontaneous EPSC (n). Dates (for example, 70 days) indicated were from the start of differentiation. AP, action potential; sEPSC, spontaneous excitatory postsynaptic currents.

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