Figure 6: Mitochondrial DNA levels and protein expression in the four lines of induced pluripotent stem cell (iPSC)-derived neurons. | Nature Communications

Figure 6: Mitochondrial DNA levels and protein expression in the four lines of induced pluripotent stem cell (iPSC)-derived neurons.

From: Parkin controls dopamine utilization in human midbrain dopaminergic neurons derived from induced pluripotent stem cells

Figure 6

(a–c) Quantitative PCR measurement of three different segments of mitochondria DNA against the nuclear gene actin in the four lines of iPSC-derived neurons treated with or without CCCP (10 μM for 16 h). mtDNA3130-3301 is a region not transcribed and has no homology with mitochondrial pseudogenes in the nuclear genome. (d) Protein expression levels of PINK1 and DJ-1 in the absence or presence of CCCP treatment (10 μM for 24 h). PINK1 has two bands, uncleaved at 63 kDa and cleaved at 52 KDa. (e) The expression level of α-synuclein in the four lines of iPSC-derived neurons. Error bars represent s.e.m.

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