Figure 4: Hes5∷CreER^T2 NSCs generate granule neurons.

(a) TAM induction regime once per day for 10 consecutive days before the mice were killed (*) 100 days (d100) after the end of induction. Quantification of labeled cells on d100, NeuN⁺ neurons are the largest single rGFP⁺ population. (b) 100 days after induction, new neuroblasts (Dcx⁺; arrows) are still being generated, indicating labeling of NSCs. (c,d) rGFP⁺NeuN⁺ neurons are found in clusters, and migrate into the granule cell layer (arrows). (e) rGFP⁺NeuN⁻ cells remain in the SGZ 100d after TAM induction (arrowhead) in close proximity to newly generated rGFP⁺NeuN⁺ neurons (arrows). (f) Consistent with having labeled NSCs, rGFP⁺ cells accumulate over the 100d of lineage tracing. (g) Lineage and population development of Hes5∷CreER^T2-derived rGFP⁺ cells over time. (h) Graphical representation of the development, production and maintenance of the individual Hes5∷CreER^T2-derived cell types in the DG lineage (absolute numbers). (h′) Representation of the area indicated in (h) showing the development of proliferating cells (PCNA) in the DG lineage relative to Ascl1 (type-2a)-, Tbr2 (type-2b)- and Dcx (neuroblast)-expressing cells (absolute numbers). GCL, granule cell layer; ML, molecular layer; SGZ, subgranular zone; TAM, tamoxifen. Error bars are s.d. Scale bars in (b) and (c) equals 100 μm, in (d) and (e) equals 10 μm.