Figure 2: TAF are persistent following H2O2- and neocarzinostatin-induced senescence in MRC5 fibroblasts. | Nature Communications

Figure 2: TAF are persistent following H2O2- and neocarzinostatin-induced senescence in MRC5 fibroblasts.

From: Telomeres are favoured targets of a persistent DNA damage response in ageing and stress-induced senescence

Figure 2

(a) Mean number of both TAF and non-TAF in MRC5 fibroblasts at 3 and 8 days after treatment for 1 h with hydrogen peroxide (400 μM in serum-free media). Data are mean±s.e.m., n=15. (b) Mean number of both TAF and non-TAF in MRC5 fibroblasts at 3 and 8 days after treatment for 1 h with neocarzinostatin (80 ng ml−1). Mean±s.e.m.; n=15. (c) Representative images of Sen-β-Gal staining in non-treated (NT) MRC5 fibroblasts and 10 days after 1 h treatment with NCS (80 ng ml−1) and H2O2 (400 μM) (light blue-DAPI; darker cytoplasmic blue- Sen-β-Gal). (d) Representative images of Ki67 staining in non-treated (NT) MRC5 fibroblasts and 10 days after 1 h treatment with NCS (80 ng ml−1) and H2O2 (400 μM). (e) Quantification of mean number of Sen-β-Gal-positive cells and (f) Ki67-positive nuclei. Data are mean±s.e.m. (n=3). Scale bar: 20 μm.

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