Figure 3: The distance between the distal and proximal C-T measured using transition-metal ion FRET.
From: Perturbation of sodium channel structure by an inherited Long QT Syndrome mutation
(a) Model of the proximal (EF-hand motif) C-T is shown as in Figure 2. The H6 is shown on the right with the three di-histidine pairs: H6-N, H6-M and H6-C indicated with blue, red and green side chains, respectively. (b) Ni2+-dependent quenching of H6-N (blue), H6-M (red) and H6-C (green; Table 1). (c) Distances of H6-N, H6-C and H6-M measured using transition-metal ion FRET. Scale bars represent mean distance±s.d. of H6-N (n=4), H6-M (n=7) and H6-C (n=5).
