Table 1 Summary of FRET data.

From: Perturbation of sodium channel structure by an inherited Long QT Syndrome mutation

Construct

E

R

n

WT

0.04±0.04

NA

4

H4 (F1855H/K1859H)

0.12±0.01

16.7±0.3

6

H6-N (H1900/V1903H)

0.49±0.04

12.1±0.3

4

H6-M (M1906H/R1910H)

0.24±0.03

14.5±0.3

7

H6-C (H1915/Q1918H)

0.31±0.02

13.7±0.2

5

S1904L H6-N

0.14±0.03

16.3±0.6

4

S1904L H6-M

0.01±0.02

NA

8

S1904L H6-C

0.10±0.02

17.5±0.5

4

  1. FRET efficiency (E) was calculated from equation (1) in Methods. The distance (R) between the fluorescence donor (W1798) and acceptor (Ni2+) was calculated using equation (2), the Förster equation with a Förster distance (R0) of 12 Å. Values are reported as mean±s.d.; n is the number of experiments.
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