Figure 1: End-point sPMCAb titration.
From: Fast and ultrasensitive method for quantitating prion infectivity titre
10% scrapie brain material from SSLOW- (a) or 263K-infected animals (b,c) were diluted in tenfold serial steps, then aliquots from each dilution were used to seed serial PMCAb reactions. Example of a western blot of serial PMCAb (sPMCAb) products amplified for six rounds is shown in plots a and b. Panel c shows products of PMCAb reactions seeded with four dilutions and amplified for 2, 3 or 6 serial rounds. The dilutions shown for PMCAb reactions were normalized per gram of brain tissue, to correct for differences in assay volumes used for PMCAb (100 μl) and bioassay (50 μl).
