Figure 2: Expression patterns of TAD1.
From: Degradation of MONOCULM 1 by APC/CTAD1 regulates rice tillering
(a) Flow cytometric analysis of the cell-cycle profiles of the wild type (WT) and mutant (tad1). Nuclei released from the wild-type and tad1 suspension cells were stained with 4,6-diamidino-2-phenylindole (DAPI) for flow cytometry analysis. The percentage of cells in each phase of the cell cycle was quantified by the ModFit LT software. Values are means with s.e. (n=4). The double asterisks indicate the significant difference determined by the two-tailed t-test at P<0.01. (b)TAD1 transcript levels in various organs, including roots (R), shoot apexes of seedlings (SA), axillary buds (AB), internodes (I), nodes (N), young leaves (L) and young panicles (P). Values are means with s.d. of three independent experiments. (c–h) TAD1 expression patterns revealed by messenger RNA in situ hybridization. YL, young leaf; TB, tiller bud; IF, inflorescence; SA, shoot apex; CR, crown root; V, vascular bundle. Arrows in (c–g) indicate the TAD1 expression sites. (h) The in situ hybridization result with TAD1 sense probe. Scale bars, 500 μm.
