Figure 4: Determination of the interaction between TAD1 and MOC1 by coimmunoprecipitation and BiFC assays.
From: Degradation of MONOCULM 1 by APC/CTAD1 regulates rice tillering
(a) The conserved D-box motif at the MOC1 N-terminal and its mutated construct (mMOC1). (b) In vivo interaction between TAD1 and MOC1 revealed by the coimmunoprecipitation assay. (c) BiFC analysis of interaction between MOC1 and TAD1 in rice protoplasts. Scale bars, 10 μm. (d) Schematic representation of the truncated TAD1 proteins. Blue box refers to C-box, green to CSM, pink to WD repeats, white to RVL, and red to IR. (e) BiFC analyses of in vivo interaction between MOC1 and truncated TAD1 proteins indicated in (d). Scale bars, 10 μm.
