Figure 4: The effects of EGCG on TDP-43. | Nature Communications

Figure 4: The effects of EGCG on TDP-43.

From: The self-interaction of native TDP-43 C terminus inhibits its degradation and contributes to early proteinopathies

Figure 4

(a) Stimulation with 20 μM EGCG increased the FL of TDPSupN*. The TDPSupN*-expressing cells were treated with or without EGCG for 3 days and were then harvested for analysis using anti-GFP antibodies. *FL (n=3). (b) A biochemical analysis of endogenous proteins with or without EGCG using anti-TDP-43 antibodies (n=3). *P<0.05 by analysis of variance. (c) EGCG attenuated the protein degradation of GFP-Q303P-TDP-43. (d) Upon EGCG addition, GFP-Q303P-TDP-43 aggregates (arrow) appeared by using anti-GFP antibodies. Scale bar represents 10 μm. (e) The treatment of PLD truncated mTDP-43 mutant with EGCG. Scale bar represents 10 μm. (f) MTT assay of viability of TDP-43-FL-expressing cells given EGCG (n=5). (g) A biochemical analysis of GFP-TDP-43-FL proteins with or without EGCG using anti-GFP antibodies. (h) A biochemical analysis of endogenous proteins with or without EGCG using anti-TDP-43 antibodies. Data are presented as means (n=2).

Back to article page