Figure 2: Systematic approach for mammalian organelle targeting. | Nature Communications

Figure 2: Systematic approach for mammalian organelle targeting.

From: Combinatorial targeting and discovery of ligand-receptors in organelles of mammalian cells

Figure 2

(a) Organelle targeting and random peptide iPhage library selection flow diagram. (b) Phage displaying the mitochondrial localization signal of cytochrome c oxidase (MLS-iPhage) in the pIII protein is enriched in the mitochondria/ER fraction. Cytosol and nuclear fractions served as internalizing and negative controls, respectively. Bars represent mean values for TU recovered from the mitochondria/ER fraction±s.e.m., from triplicates. (c) Confocal fluorescence microscopy analysis of KS1767 cells exposed to parental phage, iPhage and MLS-iPhage. Intracellular localization was revealed by anti-rabbit Alexa Fluor 488, orange-Mitotracker, and DAPI counterstaining. Scale Bar, 10 μm. Arrows indicate signal co-localization.

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