Figure 3: Synchronous selection of a random peptide iPhage library is enriched in the mitochondria/ER fraction.
From: Combinatorial targeting and discovery of ligand-receptors in organelles of mammalian cells
(a) Systematic approach of selection in live KS1767 cells. (b) Phage enrichment after three rounds of selection. Bars represent mean values for phage TU recovered from the mitochondria/ER enriched fraction±s.e.m., from triplicates. (c) Bright-field microscopy of KS1767 cells infected with different peptide-iPhage clones selected from the mitochondria/ER fraction. Scale bar, 100 μm. Cell viability was measured using two independent methods (d) WST-1 and (e) MTT assays. Bars represent mean values of cell viability±s.e.m., from triplicates. The symbol * indicates significant reduction of cell viability (P<0.02). Data were statistically analysed by Student's t-test.
