Figure 4: RPL29 is the receptor for the internalizing YKWYYRGAA peptide.
From: Combinatorial targeting and discovery of ligand-receptors in organelles of mammalian cells
(a) Phage-binding assay on fractions from YKWYYRGAA affinity chromatography. Fractions were immobilized in a 96-well plate. Insertless iPhage and BSA were used as negative controls. Phage binding assays were run in triplicate; bars represent mean values for phage TU recovered from immobilized proteins±s.e.m., from triplicates. (b) RPL29 was identified by mass spectrometry. Amino acids in yellow represent the peptides detected by mass spectrometry. (c) Phage binding to immobilized GST–RPL29 and GST–RPL30 fusion proteins. BSA or GST alone was used as negative controls. Bars represent mean values for phage TU recovered from immobilized proteins±s.e.m., from triplicates. (d) Binding of the YKWYYRGAA-phage to RPL29 was inhibited with the corresponding synthetic peptide. Bars represent mean±s.e.m., from triplicates.
