Figure 6: The YKWYYRGAA-pen peptide induces ultrastructural alterations leading to cell death.
From: Combinatorial targeting and discovery of ligand-receptors in organelles of mammalian cells
(a) Electron microscopy analysis of KS1767 cells untreated (media) and treated with peptides (30 ÎĽM) at different time points (0.5, 1, 2, and 4 h). The cells treated only with the YKWYYRGAA-pen peptide suffered ultrastructural alterations as early as 30 min; the plasma membrane and the cytoplasm were extensively dismantled (red arrows) compared with cells treated with peptide controls or media. Scale bar, 2 ÎĽm. (b) Intracellular compartments analysis by transmission electron microscopy shows normal-vesicular (black arrow) to swollen mitochondria (red arrow), such structural alterations were only observed in KS1767 cells treated with YKWYYRGAA-pen peptide (30 ÎĽM). Normal mitochondria were observed in KS1767 cells treated with control peptides (30 ÎĽM) and media. Scale bar, 500 nm.
