Figure 7: MHF–FANCM physical contact is essential for a stable localization of FANCM to chromatin.
From: The structure of the FANCM–MHF complex reveals physical features for functional assembly
(a) MHF1 is essential for a stable localization of FANCM to centromere. GFP–FANCM is readily apparent at the centromere in the scramble short interfering RNA-treated cells (upper panel merge). GFP–FANCM failed to localize to the centromere in the absence of MHF1 (lower panel). (b) (MHF1–MHF2)2 tetramer is essential for a stable FANCM association with chromatin. (c) Schematic diagram of structure perturbation in disease-associated mutant FANCMS724X. (d) Stable MHF–FANCM-F association requires a physical contact between MHF and FANCM-F. GST pull-down results show that MHF binds to FANCM-F (FANCM661−800) (lane 1), but not FANCM661−730 (lane 2). (e) Perturbation of MHF–FANCM-F association abrogates the localization of FANCM to chromatin. GFP–FANCM is readily apparent at the centromere as it co-localizes with ACA and MHF1 (upper panel). GFP–FANCM1−723 failed to localize to the centromere (lower panel).
