Figure 6: sAPP-α inhibits amyloidogenic APP processing in vivo.
From: Soluble amyloid precursor protein-α modulates β-secretase activity and amyloid-β generation
Mouse brain homogenates were prepared from 8-month-old PSAPP and PSAPP/TgsAPP-α mice. (a) Total soluble and insoluble Aβ40, 42 were biochemically assessed in brain homogenates by ELISA and (b) total soluble Aβ species from brain homogenates were analysed by IB using 6E10 antibody. For (a), data are presented as picograms (soluble Aβ40,42) or nanograms (insoluble Aβ40,42) per milligrams of protein (mean±s.d.; n=5 females per group). Mouse brain homogenates (200 μg) per mouse were IP with (c) an anti-C-terminal BACE1 antibody for BACE1, followed by IB analysis with pAb751/770 (C-APP) for full-length APP (holo APP) (d) with an anti-C-terminal BACE1 antibody for BACE1, followed by IB for sAPP-α with 2B3 antibody. As indicated below this figure, IB analyses demonstrate total protein levels of around 100 kDa total APP by 6E10 antibody and 75 kDa BACE1 in the different mouse brain homogenates. All results as presented are representative of results obtained for five mice per group (*P<0.05, ***P<0.001).
