Figure 3: Nociceptive responses of different tissue-specific Nav17 KO mice.
From: Distinct Nav1.7-dependent pain sensations require different sets of sensory and sympathetic neurons
Littermate (white columns), Nav1.7Nav1.8 (blue columns), Nav1.7Advill (red columns) and Nav1.7Wnt1 (green columns), N shown as littermate/Nav1.7Nav1.8/Nav1.7Advill/Nav1.7Wnt1. (a) Randall–Selitto test (noxious mechanical stimulus; N=23/6/13/5). (b) Acetone cooling test (noxious cooling stimulus; N=29/6/7/5). (c) Hargreaves' test (spinal noxious heat stimulus; N=22/6/7/7). (d) Hotplate test (supraspinal noxious heat stimulus; N=37/8/19/10): in vivo electrophysiological spinal cord recording from Nav1.7Advill (red boxes) and littermate (white boxes) mice. (e) Thermally evoked WDR responses (N=6 per group). (f) Mechanically evoked WDR responses (N=6 per group). (g) Hotplate test following 6-OHDA treated (purple column, N=10) and untreated (white column, N=12) C57/Black6 mice. (h) Hotplate test of Nav1.7Advill (red column, N=7) and littermate mice (white column, N=12) following chemical 6-OHDA treatment. (a–d) Data analysed by t-test, (e–h) data analysed by two-way analysis of variance followed by the Bonferroni post hoc test. Results are presented as mean±s.e.m. ***P<0.01 and ***P<0.001 (individual points).
