Figure 4: Behavioural responses of different tissue-specific Nav1.7 and Nav1.8 KO mice to cooling and extreme cold.
From: Distinct Nav1.7-dependent pain sensations require different sets of sensory and sympathetic neurons
N shown as littermate/Nav1.7Wnt1. (a) Nav1.7Wnt1 (green columns) and littermate (white columns) core (N=12/13) and skin temperature (N=12/9). (b) Behavioural response of Nav1.8 KO (turquoise column, N=5) and littermate (white column, N=7) mice to the acetone cooling test. (c) Behavioural response of Nav1.8 KO (turquoise columns, N=8) and littermate (white columns, N=6) thermal place preference test. (d–f) Littermate (white columns), Nav1.7Nav1.8 (blue columns), Nav1.7Advill (red columns), Nav1.7Wnt1 (green columns) N shown as littermate/Nav1.7Nav1.8/Nav1.7Advill/Nav1.7Wnt1. (d) Behavioural response of all three Nav1.7 tissue-specific knockout mice to thermal place preference test (N=15/8/8/8). (e) Behavioural response of all three Nav1.7 tissue-specific knockout mice to the von Frey test. (f) Behavioural response of all three Nav1.7 tissue-specific knockout mice to the Rotarod test (N=23/8/13/9). (a,b) Data analysed by t-test, (c–f) data analysed by two-way analysis of variance followed by the Bonferroni post hoc test. Results are presented as mean±s.e.m. **P<0.01 (individual points).
