Figure 1: Characterization of KAN-1 and AvianPr reassortant viruses in cell culture and mice.
From: Adaptive mutations in NEP compensate for defective H5N1 RNA replication in cultured human cells
(a) Amino-acid differences between indicated viral proteins of KAN-1 and the avian precursor AvianPr. (b, c) Growth curves of infected cell culture. Confluent canine MDCKII (b) and avian LMH (c) cells were infected with an MOI of 0.001 and incubated at 37 °C. At the indicated time points post infection virus titre in the supernatant was analysed by plaque assay. Error bars indicate the standard error of the mean of three independent experiments. Student's t-test was performed to determine the P value. ***P<0.001. (d) LD50 of KAN-1, AvianPr, and the indicated AvianPr reassortant viruses after intranasal infection of BALB/c mice (n=5 per group). Reassortant viruses include AvianPr harbouring either all three polymerase segments (AvianPr-3P-KAN-1), NS (AvianPr-NS-KAN-1), NA (AvianPr-NA-KAN-1) or both the NS and polymerase segments (AvianPr-3P/NS-KAN-1) of KAN-1. Segments originating from KAN-1 virus are highlighted in grey.
