Figure 3: The capacity of GY118F to sustain self-renewal is dependent on STAT3.

(a) Phase images of GY118F Stat3+/+ and Stat3−/− ES cells switched to N2B27 plus G-CSF. Empty vector transfectants were used as a control. White bars correspond to 123 μm. (b) qRT–PCR analysis of GY118F Stat3+/+ ES cells cultured in N2B27 plus G-CSF for pluripotency markers genes. Error bars indicate ±1 s.d. (n=3).