Figure 7: Activation of JAK/STAT3 is dominant over FGF signalling for Epiblast stem cell reprogramming to naive pluripotency. | Nature Communications

Figure 7: Activation of JAK/STAT3 is dominant over FGF signalling for Epiblast stem cell reprogramming to naive pluripotency.

From: JAK/STAT3 signalling is sufficient and dominant over antagonistic cues for the establishment of naive pluripotency

Figure 7

(a–d) Reprogramming of GY118F EpiSCs in N2B27 plus FGF2 and G-CSF and subsequent analysis. (a) Phase and fluorescent images showing the emergence of Oct4-GFP-positive colonies and AP staining of GY118F cells. Puromycin selection was applied during 5 days prior AP staining to eliminate Oct4-GFP negative cells. White bars correspond to 161.4 μm. (b) Phase and fluorescent images of derived GY118F Oct4-GFP-positive cells maintained in N2B27 plus FGF plus G-CSF. White bars correspond to 161.4 μm. (c) qRT–PCR analysis for naïve pluripotency and EpiSC marker genes. An iPS cell line cultured in 2i plus LIF was used as a control for naïve pluripotent gene expression. Gene expression and associated error bars representing s.d. (n=3) were normalized to EpiSCs. (d) Agouti coat colour shows contribution of GY118F iPS cells derived and maintained in N2B27 plus FGF and G-CSF.

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