Figure 7: Evidence for α-helicity of the linker region by CD.

(a) CD spectrum of the mHv1/VSOP-cc harbouring the linker peptides at 4 °C. The amino-acid sequences of the linker region are shown in Fig. 4a and Supplementary Fig. S9. (b) Helix contents estimated by CD spectrum. Each two data sets were obtained for the protein constructs. CC is the crystal construct (the mHv1/VSOP-cc protein). Details of the protein constructs analysed are indicated in Supplementary Fig. S9b,f. (c) Melting temperature analysis of the coiled-coil proteins harbouring the linker peptides. Temperature dependence of the CD signal recorded from the coiled-coil proteins harbouring the linker peptides at 222 nm.