Figure 7: Expression of Hoxa genes is reduced in mouse Tet2−/− tissues.
From: Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster
(a) qRT–PCR expression analysis of the eleven Hoxa and three Tet genes in murine wt tissues (indicated above the columns). The heat map shows average Hoxa and Tet expression as percentage of the internal GPDH levels. Expression patterns of both groups of genes in 13.5-day wt embryos is shown as a positive control (most left column). Four examples of tissues with very low or absent expression are also shown (chest, muscle, liver and heart). (b–(d) qRT–PCR expression analysis of Hoxa genes that showed significant levels in (a) and the three Tet genes in selected Tet2−/− tissues. Histograms show the expression as fold change relative to the wild type (=1). For Tet2, two different primer pairs were used39. (b) Brain, (c) kidney, (d) spleen and (e) lung. s.d.'s of three replicates are indicated by error bars.
