Figure 8: Levels of 5mC and 5hmC at selected regions of the murine Hoxa cluster in control and Tet2−/− tissues.
From: Hydroxylation of 5-methylcytosine by TET2 maintains the active state of the mammalian HOXA cluster
Genomic DNA was isolated from three tissues showing significant Hoxa expression in the wt situation (kidney, spleen and lung) and used for (h)MeDIP analysis with antibodies specific for 5mC and 5hmC. (a) Diagram showing the mouse Hoxa transcription units on chromosome 6 (in dark blue), the CGIs within the cluster (green squares) and the four (h)MeDIP-amplicons (indicated as black lines) used for the analysis. Genomic features are viewed as custom tracks in the UCSC genome browser55. The following regions were analysed: (b) mHoxa2 promoter, (c) mHoxa4 first exon, (d) mHoxa5 first exon and (e) mHoxa7 promoter. The diagrams show the distribution of 5mC (left in each panel) and 5hmC (right in each panel) at four regions of the mouse Hoxa cluster in three tissues (kidney, spleen and lung) in wt (black bars) and Tet2−/− (grey bars). Enrichments were calculated relative to the unmethylated mGapdh and mbeta-actin controls. s.d.'s of three replicates are indicated by error bars.
