Figure 8: Characterization of MVSCs from neointima in rat and MVSCs from normal human carotid artery.
From: Differentiation of multipotent vascular stem cells contributes to vascular diseases
(a) Alcian blue staining of a cross-section of carotid artery from SD rat at week 5 after injury. Square indicates the area where the cells were isolated. Scale bar, 50 μm. (b,c) The cells isolated from neointima in rat were stained for MVSC markers Sox10 and Sox17. Scale bars, 50 μm. (d–i) The cells isolated from neointima were subjected to differentiation assays and stained for SMC markers SM-MHC and CNN1 (d), Schwann cell markers GFAP and S100β (e), neuronal marker TUJ1 and peripherin (f), aggrecan in chondrogenic culture using alcian blue (g), oil droplets in adipogenic culture using oil red (h), and calcified matrix in osteoblastic culture using alizarin red) (i). Scale bars of (d–f), 50 μm. Scale bars of (g–i), 100 μm. (j–o) Immunostaining of MVSCs isolated from human carotid arteries with antibodies against Sox10, Sox17, Pax-3/7, vimentin, NFM and S100β. Scale bars, 100 μm.
