Figure 1: Skeletal muscle stem cells survive for extended periods post mortem.
From: Skeletal muscle stem cells adopt a dormant cell state post mortem and retain regenerative capacity
Tissue and cells from freshly sampled human biopsy (a–c). (a) Histology. Insets: immunostaining with satellite cell marker (CD56, brown). (b) Myotubes in vitro after 14 days culture from muscle biopsy (from same patient) expressing myogenin and desmin (c). Human deltoid muscle collected 17 days post mortem (d–f), (d) histology— inset is a CD56+ satellite cell, (e) myotube formation expressing myogenic markers (f). Muscle biopsy stored for 25 days post sampling (ps) from patient (g–i), (g) histology with a CD56+ satellite cell (inset), (h) myotube formation expressing myogenic markers (i). Mouse skeletal TA muscle 8 days post mortem (j–l) (n=10 mice per time point). (j) Histology—inset satellite cell expressing M-Cadherin, (k) myotube formation, expressing myogenin and desmin (l). Note necrosis of haematoxylin and eosin (H&E)-stained myofibres (d–j). Histology figures are H&E stainings. Myotubes are stained with May Grünwald Giemsa (MGG) and immunostained with anti-myogenin (green), anti-desmin (red), and nuclei are counterstained with Hoechst (blue). Bar: 10 μm.
