Figure 5: Muscle stem cells that survive post mortem or in anoxia retain robust regenerative potential after transplantation.
From: Skeletal muscle stem cells adopt a dormant cell state post mortem and retain regenerative capacity
(a–c) Transplantation of muscle stem cells from Tg:Pax7-nGFP::Tg:CAG-PLAP::Tg:MLC3F-nlacZ-2E donor mice into pre-injured TA muscle of immunocompromised recipient mice (n=5 mice). Immunohistochemistry showing contribution of donor-transplanted stem cells to regenerating fibres (red) in recipient mouse; inset in b shows a satellite cell in the niche after self-renewal. (d) Number of myofibres engrafted in the recipient muscle after injection of 5000 freshly isolated, post mortem, or anoxic donor cells (n=5 recipient mice each; 54±17 PLAP positive fibres with freshly isolated cells, 75±26 with 4 days post mortem cells, 69±29 with anoxic donor cells). (e) Distribution of the number of capillary sections around myofibers after regeneration in non-engrafted regenerated fibers (centrally nucleated), engrafted (hPLAP-positive) with 4 days post mortem or 4 days post anoxia donors. Statistical analysis was performed using paired or unpaired Student's t- tests, a minimum of 95% confidence interval for significance. Figures display average values of all tests ±s.e.m.
