Figure 7: FIGIRKO mice are extremely cold sensitive.

(a) Rectal temperature was measured in 4 month old control and FIGIRKO male mice every 30 min for 2 h after exposure to a 4 °C environment (n=9 per group). (b) UCP1 mRNA abundance was measured by real-time PCR in PG, SC and BAT in 4 month old male control and FIGIRKO mice. (c) mRNA abundance was measured by real-time PCR in BAT from 4 month old control and FIGIRKO male mice. Results are mean±s.e.m. of 6 animals/group. (d) Control and FIGIRKO mice were anesthetized and injected with 18-FDG. One hour after FDG injection, whole body PET-CT scans were performed and the interscapular area was analysed to measure FDG uptake in BAT. The experiment was repeated 1 h after injection of CL316243 (1 μg/g body weight) intraperitoneally. Results are mean±s.e.m. of 2 animals/group. Right panel show three-dimensional reconstruction images of control and FIGIRKO mice after FDG injection in the basal state. (e) UCP1, PGC1α and Elovl3 mRNA abundance was measured by real time PCR in PG, SC and BAT in 4 month old control and FIGIRKO male mice injected with either saline or CL316243 (1 μg/g body weight) intraperitoneally. Results are mean±s.e.m. of 5 animals/group and are expressed as fold change over the saline group. Statistical significance assessed by two-tailed Student's t-test, *P<0.05 between control and FIGIRKO mice, #P<0.05 between saline and CL316243 treated group.