Figure 5: Trim71 domains required for P-body localization.

(a) KH2 ESCs transfected with a DCP1a::GFP construct are fixed and probed with α-Trim71 antibody. Nuclei are stained with DAPI. (b) HeLa cells are co-transfected with DCP1a::GFP and mCherry::Trim71 fusions. (c) Individual mCherry::Trim71 constructs were co-transfected with DCP1a::GFP into HeLa cells and visualized by fluorescence microscopy. Scale bar, 5 μm.