Figure 1: Increased histone acetylation increases H1 dynamics. | Nature Communications

Figure 1: Increased histone acetylation increases H1 dynamics.

From: Histone modifications and lamin A regulate chromatin protein dynamics in early embryonic stem cell differentiation

Figure 1

(a–d) FRAP analysis (± s.d.) of H1e–GFP in euchromatin (a,c) and heterochromatin (b,d) in MEFs (a,b) and in R1 mouse ESCs (c,d) treated (grey) or untreated (black) with the HDAC inhibitor VPA for 24 h. Changes for euchromatin are significant (a, P<0.005; c, P<0.01; d, P>0.05, 2-tailed t-test). (e, f) FRAP analysis of H1e–GFP in euchromatin of MEFs treated (black) or untreated (grey) with TSA (e) or VPA (f) for 4 h. Changes are significant (P<0.005 in both cases, 2-tailed t-test). (g,h) FRAP analysis of H1e–GFP in euchromatin of mouse ESCs treated (black) or untreated (grey) with TSA (g) or VPA (h) for 4 h. Changes are significant (P<0.05 in both cases, 2-tailed t-test). (i,j) FRAP analysis of H1e–GFP in heterochromatin of MEFs treated (black) or untreated (grey) with TSA (i) or VPA (j) for 4 h. (k,l) FRAP analysis of H1e–GFP in heterochromatin of ESCs treated (black) or untreated (grey) with TSA (k) or VPA (l) for 4 h. Values are from at least 2 independent experiments, at least 20 cells each. Error bars represent s.d.

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