Figure 7: DNA methylation does not affect H1 dynamics in ESCs. | Nature Communications

Figure 7: DNA methylation does not affect H1 dynamics in ESCs.

From: Histone modifications and lamin A regulate chromatin protein dynamics in early embryonic stem cell differentiation

Figure 7

(a) H1e–GFP FRAP analysis in euchromatin of R1 ESCs, treated for 5 days with either 100 mM 5-Aza (grey) or 1 mM Zeb (empty) or untreated (Ct, black). (b) H1e–GFP FRAP analysis in euchromatin of Dnmt1 knockout ESCs (Dnmt1−/−, grey) compared with wild-type controls (WT, black). (c) H1e–GFP FRAP analysis in euchromatin of Dnmt3a/b double-knockout ESCs (Dnmt3−/−, grey) compared with wild-type controls (WT, black). (d) H1e–GFP FRAP analysis in euchromatin of Dnmt3a/b and Dnmt1 triple-knockout ESCs (Dnmt1/3-TKO, grey) compared with wild-type controls (WT, black). Changes are not significant (P=0.066, 2-tailed t-test). (e) H1e–GFP FRAP analysis in heterochromatin of R1 ESCs, treated for 5 days with either 100 mM 5-Aza (grey) or 1 mM Zeb (empty) or untreated (Ct, black). (f) H1e–GFP FRAP analysis in heterochromatin of Dnmt1 knockout ESCs (Dnmt1−/−, grey) compared with wild-type controls (WT, black). (g) H1e–GFP FRAP analysis in heterochromatin of Dnmt3a/b double-knockout ESCs (Dnmt3, grey) compared with wild-type controls (WT, black). (h) H1e–GFP FRAP analysis in heterochromatin of Dnmt3a/b and Dnmt1 triple-knockout ESCs (Dnmt1/3-TKO, grey) compared with wild-type controls (WT, black). Changes are not significant (P=0.07, 2-tailed t-test). Values are from at least 2 independent experiments, at least 20 cells each.

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