Figure 8: Nucleosome spacing does not affect chromatin protein dynamics in ESCs. | Nature Communications

Figure 8: Nucleosome spacing does not affect chromatin protein dynamics in ESCs.

From: Histone modifications and lamin A regulate chromatin protein dynamics in early embryonic stem cell differentiation

Figure 8

(a) H1e–GFP FRAP analysis in euchromatin of H1c/d/e triple-knockout ESCs (H1c/d/e−/−, grey) compared with wild-type controls (WT, black). (b) HP1–GFP FRAP analysis in euchromatin of H1c/d/e triple-knockout ESCs (grey) compared with wild-type controls (black). (c) HMGN1–GFP FRAP analysis in euchromatin of H1c/d/e triple-knockout ESCs (grey) compared with wild-type controls (black). (d) H1e–GFP FRAP analysis in heterochromatin of H1c/d/e triple-knockout ESCs (grey) compared with wild-type controls (black). (e) HP1–GFP FRAP analysis in heterochromatin of H1c/d/e triple-knockout ESCs (grey) compared with wild-type controls (black). (f) HMGN1–GFP FRAP analysis in heterochromatin of H1c/d/e triple-knockout ESCs (grey) compared with wild-type controls (black). No significant changes were observed for any of the tested proteins. Values are from at least 2 independent experiments, at least 20 cells each.

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