Figure 4: CAS regulates salicylic acid (SA) biosynthesis and its related gene expression.
From: Chloroplast-mediated activation of plant immune signalling in Arabidopsis
(a–e) Flg22-induced phytohormone accumulation was measured at the indicated time points in leaves of wild-type (black line) and cas-1 knockout (red line) plants. Data are the means±s.e.m. of three experiments. (a) SA and (b) jasmonic acid (JA) accumulation were measured using UPLC/TOFMS, and (c) abscisic acid (ABA), (d) indole-3-acetic acid (IAA) and (e) cytokinins were measured using nano-LC/MS/MS, and has been shown as the amount of hormones per fresh weight (FW) of plants. (f) Flg22-induced gene expression was analysed at indicated time points by qRT–PCR. ACT7 was used as an internal standard. Data are mean±s.e.m. of three experiments.
