Figure 5: PIN8 and PIN5 act antagonistically. | Nature Communications

Figure 5: PIN8 and PIN5 act antagonistically.

From: ER-localized auxin transporter PIN8 regulates auxin homeostasis and male gametophyte development in Arabidopsis

Figure 5

(a) Morphological defects in pollen of pin5 (2,400) and pin8 (2,400) single mutants were rescued in pin5 pin8 (3,300) double mutants as shown by DAPI staining. A total of 7,100 wild-type (WT) pollens were counted as the control. Error bars represent the standard error of more than 23 independent plants (Student's t-test, **P<0.01). (b) The reduced pollen transmission ability in the pin8 mutant was rescued in the pin5 pin8 double mutant. Error bars represent the standard error of more than ten independent crosses (Student's t-test, *P<0.5). (c,d) The long hypocotyl phenotype of the PIN8OX line was rescued in the PIN8OX PIN5OX line. Error bars represent the standard error of three independent experiments (n=60, Student's t-test, **P<0.01). (e) Specific HPLC chromatogram of IAA metabolic profile of PIN8OX was largely rescued in the PIN8OX PIN5OX line (2.5-h incubation with 3H-IAA). Arrows indicate the notable changes in the IAA metabolic profile. (f) The decreased free IAA-Asp of the PIN8OX line was rescued in the PIN8OX PIN5OX line. Error bars represent the standard error of the mean, n=3 (Student's t-test, *P<0.05).

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