Figure 2: Local and systemic IL-17 upregulation in PLCδ1−/− mice.
From: Epidermal phospholipase Cδ1 regulates granulocyte counts and systemic interleukin-17 levels in mice
(a) IL-17 concentration in the serum measured by ELISA. Mean±s.e.m. (n=6). (b) Representative FACS profiles of intracellular IL-17 in ILNs and MLNs. Cells were stimulated and intracellular IL-17 was detected (n=6 for ILNs and n=3 for MLNs). Three independent experiments were performed. (c) Absolute numbers of IL-17+ cells in ILNs. Mean±s.e.m. (n=6). The combined results from three independent experiments are displayed. (d) IL-17 mRNA expression in the ILNs determined by real-time RT–PCR. All values are normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Results are displayed as arbitrary units (expression in PLCδ1+/− mice=1). Mean±s.e.m. (n=3). (e) Representative FACS profiles of γδ-TCR and intracellular IL-17 in ILNs (n=3). Cells were stimulated and intracellular IL-17 was detected. Three independent experiments were performed. (f) IL-17 mRNA expression in the axillary lymph nodes (ALNs) determined by real-time RT–PCR. All values are normalized to GAPDH. Results are displayed as arbitrary units (expression in PLCδ1+/− mice=1). Mean±s.e.m. (n=5). (g) IL-17 mRNA expression in skin determined by real-time RT–PCR. PLCδ1−/− ILN was used as the positive control for IL-17 expression. All values are normalized to GAPDH. Results are displayed as arbitrary units (expression in PLCδ1−/− ILN=1). Mean±s.e.m. (n=5). Mice used in all experiments were 8–12 weeks old. Statistical significance was assessed using a Student′s t-test. **P<0.01. ND; not detected.
