Figure 1: Primary sequence and structure model of resilin in D. melanogaster.

(a) Hierarchical structure of fruit fly resilin. The resilin fibrils with crosslinking consist of two major unstructured peptides derived from exon I and III of full-length resilin. (b) In total, 4–12% SDS–polyacrylamide gel electrophoresis of full-length resilin (lane 1), exon I (lane 2) and exon III (lane 3) samples from purification (upper panel) and fluorescence of full-length resilin film under white (left) and ultraviolet (right) light (lower panel). The scale bar is 5 mm. Seeblue plus2 pre-stained standard (Invitrogen, CA, USA) was used as size markers. (c) Amino-acid sequence scheme of three exons in the full-length resilin protein. (d) Hydrophobicity index of the full-length resilin. (e) Proposed transmembrane segment of resilin³⁴.