Table 1 eGFP and Gb′lac2 ZFNs target sites and amino-acid sequences of designed ZF recognition helices.

From: Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases

ZFN

ZFN binding sequence

Finger 1

Finger 2

Finger 3

eGFP-R

5′-GGCATCGACttcaagGAGGACGGC-3′

TSGHLVR

DLANLNR

RPDNLAH

eGFP-L

5′-CCGTAGCTGaagttcCTCCTGCCG-3′

RQRELLR

VTHNLTR

DQSALVR

Lac2-R

5′-CCTGACGACgcacgtGATGCTGCT-3′

RSHDLLR

TTTDLRR

RRHHLQH

Lac2-L

5′-GGACTGCTGcgtgcaCTACGACGA-3′

RKMHLTV

DSSSLIR

DASALRR

  1. Each ZFNs target sites are indicated by the underlines. Fingers 1–3 show the amino acids of zinc-finger recognition helices of each ZFNs.
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