Table 2 ZFN- or TALEN-induced mutagenesis after mRNA microinjection in insects.
ZFNs/TALENs | Number of injected eggs | Somatic mutants, n (Mut/G0 nymph, %) | Fertile adults, n (adults/injected eggs, %) | Germline mutants, n (Mut/crossed adults*, %) | Reference |
Gb eGFP-ZFN-L, R | 302 | ND | 64 (22) | 10 (48) | This study |
Gb eGFP-ZFN-L or R | 74 | ND | 14 (19) | 0 | This study |
Gb Lac2- ZFN-L, R | 358 | 54 (58) | 32 (7) | 15 (47) | This study |
Gb Lac2- ZFN-L or R | 84 | 0 | 7 (8) | 0 | This study |
Gb Lac2- TALEN-L, R | 168 | 18 (17) | 35 (21) | 6 (17) | This study |
Gb Lac2- TALEN-L or R | 62 | 0 | 11 (18) | 0 | This study |
Bm BL-1 (ZFN)* | 480 | ND (72) | 173 (36) | 5–9 (9–16) | Ref.13 13 |
Dm rosy (ZFN) | ND | ND | 99 (4–30) | 41 (41) | Ref.11 11 |
Dm yellow (TALEN) | ND | 32 (44) | 109 (ND) | 33 (31) | Ref.24 24 |
