Figure 1: Wide-spread expression of TN-XXL in tissues and organs of TN-XXL transgenic mice.
From: Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model
(a) Scheme of the transgene construct with the CMV enhancer/chicken β-actin (CAG) promoter upstream, and the WPRE downstream of TN-XXL. (b) Western blot analysis of different tissue lysates using an anti-GFP antibody for the TN-XXL expression and an anti-GAPDH antibody as control. (c) Images of three newborn mice (HO, homozygous; HE, heterozygous; WT, wild-type) illuminated with bright field (monochrome), CFP (blue) and cpCitrine (yellow) fluorescence. (d) Bright field and fluorescence images of isolated organs from TN-XXL transgenic mice (right) compared with wild-type controls (left). (e) Overview of TN-XXL fluorescence (green) in frozen sections of several tissues co-stained with 4′,6-diamidino-2-phenylindole (DAPI; magenta) (Scale bars, 500 μm).
