Figure 2: TN-XXL concentration and functionality in cells of TN-XXL transgenic mice.
From: Biocompatibility of a genetically encoded calcium indicator in a transgenic mouse model
(a,b) Concentration determination of TN-XXL. (a) Calibration curve using recombinant purified TN-XXL dilutions (mean±s.d, n=4). (b) Estimation of TN-XXL protein concentrations in cells of acute slices (400 μm) from brain (Ves., blood vessels; Neur, hippocampal neurons), kidney, liver, and for low, medium (med) and high expressing fibers of muscle (M) and heart (H). (c,d) FRAP experiments in cultured MKF of TN-XXL transgenic mice. (c) Fluorescence images of a kidney fibroblast. Blow-ups show the region marked with a white rectangle in the left picture. Arrows indicate the site of the bleached spot at indicated time points after bleaching (Scale bar, 15 μm). (d) Time course of fluorescence recovery after photobleaching (mean±s.e.m., n=3). (e) Functionality of TN-XXL in cultured MKF (mean±s.e.m, n=3). Fibroblasts from TN-XXL transgenic mice and from wild-type mice acutely transfected overnight with TN-XXL were compared. (e, left) Ratio for basal calcium of transgenic (TN-XXL) and wild-type MKF (WT) expressing TN-XXL. (e, right) Comparison of average agonist evoked responses for application of ATP in fibroblasts.
