Table 1 Frequencies and absolute number of BTICs in mammosphere and adherent 2D cultures.

From: TGFβ induces the formation of tumour-initiating cells in claudinlow breast cancer

Cell lines

Cell dose

f

95% CI

P

(Total cell number in M1), absolute number of BTICs

 

1

5

10

20

25

30

50

100

    

HCC1954

 

3/4

4/4

4/4

 

4/4

4/4

4/4

1:3

1:1–1:8

0.9984

(600,000), 200,000

HCC1954-TGFβ

 

0/4

2/4

3/4

 

4/4

4/4

4/4

1:14

1:7–1:26

0.7956

(750,000), 53,571

MDA-MB-231

0/4

2/4

1/4

0/4

 

2/4

4/4

 

1:33

1:17–1:65

0.1092

(295,000), 8,939

MDA-MB-231-TGFβ

0/4

0/4

0/4

1/4

 

3/4

2/2

 

1:40

1:18–1:87

0.4619

(1,800,000), 45,000

MDA-MB-231-TGFβ+Y

 

0/4

0/4

0/4

 

0/4

0/4

1/4

1:809

1:116–1: 5627

0. 9373

(1,320,000), 1,630

MDA-MB-231-vector control

0/4

2/4

0/4

 

1/4

 

3/4

 

1:43

1:19–1:98

0.092

(500,000), 11,627

MDA-MB-231-TβRII-ΔCyt

1/4

1/4

2/4

 

1/4

 

4/4

 

1:22

1:11–1:46

0.0846

(305,000), 13,864

MDA-MB-231-TβRII-ΔCyt- TGFβ

0/4

2/4

1/4

 

1/4

 

1/4

 

1:62

1:39–1:101

0.0170*

(800,000), 12,903

MDA-MB-231-TβCA

0/4

0/4

0/4

 

2/4

 

2/4

 

1:70

1:26–1:188

0.7843

(1,400,000), 20,000

MDA-MB-231-Φ-TGF β

0/4

0/4

0/4

 

1/4

 

3/4

 

1:67

1:41–1:109

0.7568

(850,000), 12,687

MDA-MB-231-sh1NEDD9-TGF β

0/4

0/4

0/4

 

0/4

 

0/4

 

>1:364

  

(950,000), <2,610

MDA-MB-231-sh2NEDD9-TGF β

0/4

0/4

0/4

 

0/4

 

2/4

 

1:156

1:40–1:605

0.7105

(1,050,000), 6,731

Cell lines

Cell dose

f

95% CI

P

(Total cell number in a 10-cm cell-culture dish), absolute number of BTICs

 

10

50

100

200

300

500

    

HCC1954 (2D)

0/4

0/4

0/4

0/4

0/4

0/4

>1:3,784

1:26,356–1:543

0.9504

(1,400,000), <3,700

MDA-MB-231(2D)

0/4

0/4

0/4

0/4

0/4

2/4

1:2,060

1:8,035–1:528

0.659

(2,600,000), 12,621

  1. Cells from the indicated cell lines were plated at the same density (50,000 cells per ml) in 4 ml of supplemented MEBM and grown as M1 mammospheres for 7 days. HCC1954 and MDA-MB-231 cell lines were also grown in a 10 cm cell-culture dish in 2D in RPMI and DMEM 10% FBS, respectively. Single-cell suspensions from these cultures were implanted into the mammary fat pad of NSG mice. Results show the number of tumours per number of implanted mammary fat pads. BTIC frequencies per transplanted populations (f) and upper and low limits (95% CI) were calculated by limiting dilution analysis. Consistency of the fit of the data according to a single-hit model is indicated by P-values (Chi-square) >0.05 (P) (all conditions except one * complied). Total cell number in the mammosphere cultures 7 days after and their calculated absolute number of BTICs is shown.
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