Figure 3: Enzyme-triggered self-assembly at different concentrations in vitro.
From: Imaging enzyme-triggered self-assembly of small molecules inside live cells
(a) Optical images of (left) the solution of 2 (0.6 wt%, pH 7.4) and (right) the hydrogel of 3 by treating 2 (0.6 wt%) with ALP (20 U ml−1) at pH 7.4. (b) The TEM image of the negatively stained nanofibres in the hydrogel of 3. (c) The confocal fluorescent microscope image (×63 oil objective lens) shows the appearance of bright spots in the solution of 2 (500 μM, pH 7.4 in PBS buffer) with the treatment of ALP (20 U ml−1). (d) TEM image shows that a fluorescent spot shown in (c) consists of the network of nanofibres. (Scale bar, b, 100 nm; c, 25 μm; d, 100 nm.)
