Figure 2: Transgenerational effect of maternal EE2-supplemented diet. | Nature Communications

Figure 2: Transgenerational effect of maternal EE2-supplemented diet.

From: High-fat or ethinyl-oestradiol intake during pregnancy increases mammary cancer risk in several generations of offspring

Figure 2

(a,c,e) Mammary tumour incidence (%) in F1 (control: n=27, EE2: n=17), F2 (control: n=24, EE2: n=22) and F3 (control: n=23, EE2: n=25) generation female offspring of Sprague–Dawley rat mothers (F0) fed a EE2-supplemented diet or a control diet during gestation. (b,d,f) Mammary tumour multiplicity (mean±s.e.m.) in F1 (control: n=15, EE2: n=14), F2 (control: n=13, EE2: n=11) and F3 (control: n=14, EE2: n=21) generation female offspring of Sprague–Dawley rat mothers (F0) fed EE2 or a control diet during gestation. (g,h) Mammary tumour incidence (control: n=24, EE2×control: n=16, control×EE2: n=12) and tumour multiplicity (control: n=13, HF×control: n=10, control×HF: n=4) in F2 generation female outcross (EE2×Con) and male (Con×EE2) outcrosses. Significant differences versus the control group were determined as follows: log-rank test (tumour incidence), t-test (tumour multiplicty) and one-way ANOVA followed by Dunn's post-hoc test (outcross groups tumour multiplicity). P<0.05 is considered significant; exact P-values are shown in each plot.

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