Figure 3: Mammary gland morphology in HF or EE2 offspring. | Nature Communications

Figure 3: Mammary gland morphology in HF or EE2 offspring.

From: High-fat or ethinyl-oestradiol intake during pregnancy increases mammary cancer risk in several generations of offspring

Figure 3

(a) Histological depiction of the fourth abdominal rat mammary gland on PND 21 and (b) terminal end buds (TEBs; indicated by arrows). (c) Number of TEBs on PND21 in F1 (control, HF: n=5), F2 (control: n=6, HF: n=5; outcrosses: n=6) and F3 (control, HF: n=5) generation female offspring of Sprague–Dawley rat mothers (F0) fed HF or a control diet during gestation. (d) Number of TEBs on PND50 in F1 (control, HF: n=5), F2 (control: n=6, HF: n=5, outcrosses: n=5) and F3 (control: n=6, HF: n=5) generation female offspring of Sprague–Dawley rat mothers (F0) fed HF or a control diet during gestation. (e) Number of TEBs on PND21 in F1 (control: n=5, EE2: n=6), F2 (control: n=6, EE2: n=5; outcrosses: n=5) and F3 (control, EE2: n=6) generation female offspring of Sprague–Dawley rat mothers (F0) fed EE2 or a control diet during gestation. (f) Number of TEBs on PND50 in F1 (control, EE2: n=6), F2 (control, EE2: n=5; outcrosses: n=6) and F3 (control: n=5, EE2: n=6) generation female offspring of Sprague–Dawley rat mothers (F0) fed EE2 or a control diet during gestation. All values are expressed as the mean±s.e.m. Significant differences versus the control group were determined as follows: t-test (HF, EE2) and one-way ANOVA (outcross groups) followed by Holm-Sidak post-hoc test. P<0.05 is considered significant; exact P-values are shown in each plot. LN, lymph node; scale bars, 3 mm (a) and 0.5 mm (b).

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