Figure 4: Effects of maternal HF or EE2 diet on methyltransferases expression. | Nature Communications

Figure 4: Effects of maternal HF or EE2 diet on methyltransferases expression.

From: High-fat or ethinyl-oestradiol intake during pregnancy increases mammary cancer risk in several generations of offspring

Figure 4

(a–c) Dnmt1, Dnmt3a and Dnmt3b mRNA expression on PND50 in rat mammary glands of F1 (control: n=6, HF: n=5), F2 (control: n=5, HF: n=4) and F3 (control: n=5, HF: n=3) generation female offspring of Sprague–Dawley rat dams (F0) fed HF or control (Con) diet during gestation. (d–f) Dnmt1, Dnmt3a and Dnmt3b mRNA expression on PND50 in rat mammary glands of F1 (control, EE2: n=6), F2 (control: n=5, EE2: n=6) and F3 (control, EE2: n=5) generation female offspring of Sprague–Dawley rat dams (F0) fed EE2 or control diet during gestation. Quantitative real-time PCR (qPCR) and absolute quantification method was used. Dnmt1, Dnmt3a and Dnmt3b expression of each sample was normalized to rRNA 18S expression. All data are mean±s.e.m. of two independent experiments performed in triplicates. Significant differences versus the control group were determined by two-way ANOVA (after log-transform) followed by Holm-Sidak post-hoc test. P<0.05 is considered significant; exact P-values are shown in each plot.

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