Figure 2: Subcellular localization of α-L-iduronidase mRNAs in developing transgenic endosperm sections. | Nature Communications

Figure 2: Subcellular localization of α-L-iduronidase mRNAs in developing transgenic endosperm sections.

From: Production of α-L-iduronidase in maize for the potential treatment of a human lysosomal storage disease

Figure 2

Endosperm sections expressing the α- L -iduronidase gene (control and test constructs) were subjected to in situ RT–PCR to assess mRNA localization (middle images). ER-derived protein bodies (1 to 2-μm in diameter) were visualized by staining the sections with rhodamine hexyl ester (left images), a dye that preferentially stains the prolamin protein bodies of maize. α-L-Iduronidase mRNAs were in situ-labelled by Oregon Green-dUTP (middle images). In the control sections, α-L-iduronidase mRNAs were seen in both protein body–ER (yellow in the merged image) and in cisternal ER regions (green in the merged image). In contrast, α-L-iduronidase mRNAs derived from the test construct were seen exclusively in the protein body–ER region. Bar, 10 μm.

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